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Primers were purchased from Eurofins MWG Operon (Ebersberg, Germany) and used to detect the specific genes listed: Human GAPDH forward 5′-CTCCTGTTCGACAGTCAGCC-3′ reverse 5′-TGGAATTTGCCATGGGTGGA’-3 and Human MnSOD forward 5′-GTTGGGGTTGGCTTGGTTTC-’3 and reverse 5′-CATAAAGAGCTTAACATACTCAGCA-3′

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This surface, characterized by the presence of a mono-thiol compound, is non-cytotoxic and naturally occurs at millimolar concentrations in most organs