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To ensure that the compounds identified in our parent and pDC-based assays were specific inhibitors of the interaction between STX7 and Munc13-4 but did not interfere with vesicle docking and exocytosis by inhibiting Rabs–Munc13-4 complexes, ENDO compounds were counterscreened in TR-FRET assays using lysates expressing Flag–Munc13-4 and EGFP–Rab27a or EGFP–Rab11
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