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2037-A DIAGNOSTIC ACCURACY OF VCTE AND 2D-SWE IN BIOPSY-PROVEN NAFLD AND PERFORMANCE OF TWO-STEP APPROACHES TO PREDICT ADVANCED FIBROSIS Madalina-Gabriela Taru 1,2 , Dan-Corneliu Leucuta 2 , Lidia Neamti 2 , Anca Maniu 3 , Vlad Taru 4 , Bobe Petrushev 3 , Ioana Rusu 3 , Cristian Tefas 2,3 , Petra Fischer 2 , Andreea-Ioana Terec 5 , Lucia Maria Procopciuc 2 , Bogdan Procopet 2 , Horia Stefanescu 3 and Monica Lupsor-Platon 2,3 , (1)Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, Italy, (2)Luliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania, (3)Octavian Fodor Regional Institute of Gastroenterology and Hepatology, 400162 Cluj-Napoca, Romania, (4)Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, (5)Niculae Stăncioiu Heart Institute, 400001, Cluj-Napoca, Romania Background: Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) pose significant challenges to the healthcare system, and the prediction of advanced fibrosis (≥F3) is a crucial aspect in their management

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The plate was moved to a Biological Safety Cabinet after each slice was made and after complete slicing, the acute CO slices were transferred to a new 6 well plate containing CO media with PenStrep and placed in the incubator at 37 °C with 5% CO 2 and humidity [82, 84]