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In primary cortical neurons, IGFBP-3 actually potentiated the protective effect of humanin against cell death induced by amyloid-β (174), but in a contrasting study, in which acetylcholinesterase was used to induce amyloid-β aggregation and cytotoxicity, IGFBP-3 binding was found to oppose the inhibitory effect of humanin on this process (175)

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D-2HG was found to inhibit the function of DNA and histone demethylases and was associated with altered epigenetic regulation, collagen synthesis, and cell signaling [54]