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Cells were washed with PBS, detached with trypsin and centrifuged (5 min, 400 g), resuspended and covered with 5 μM working solution of MitoSOX reagent in HBSS and incubated at 37 °C for 10 min in FACS tubes

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941 However, these neuroprotective drugs often fail to achieve the expected therapeutic effect due to poor BBB permeability and short circulatory half-life

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