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Hypothiocyanous acid reductase is critical for host colonization and infection by Streptococcus pneumoniae - Journal of Biological Chemistry

J Neurochem 102: 216–230, 2007

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Cells were resuspended in lysis buffer (20 mM Tris-HCl pH 7.5, 500 mM NaCl, 20 mM imidazole, 1 mM PMSF and 1 tablet per 50 ml of EDTA-free protease inhibitor cocktail) and lysed by sonication using a Branson 450 sonifier (pulses of 10 s on, 10 s off for 20 min at 60% power)