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Further experiments confirmed the presence of vitamin E, quercetin, and cetechin, which demonstrated hepatoprotective effect, and the ROS inhibition was confirmed to be associated with antioxidant activities, thus protecting against ALI

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Passive diffusion of 4-kDa FITC-dextran (Sigma-Aldrich) was added at a concentration of 5 μM to the apical chamber in 100 μl of PBS, while the basolateral chamber contained 500 μl of PBS