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If patterns suggest sensitivity to an additive rather than a technique problem, ask your clinician about formulation alternatives and whether allergy testing would help

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Pipette 150 μl, 144 μl, 138 μl, 132 μl, 126 μl, and 120 μl of assay buffer into successive tubes or microplate wells and add 0 μl, 6 μl, 12 μl, 18 μl, 24 μl, and 30 μl of the 0.1 nmol/μl standard to the wells, respectively, to generate standards containing 0 nmol, 0.2 nmol, 0.4 nmol, 0.6 nmol, 0.8 nmol, and 1.0 nmol per 50 μl